Dr. Ronit Bar-Ness Greenstein

Dept. of Oral Biology, School of Dental Medicine

email: yoronit@post.tau.ac.il

Research topic: Oral microbiology – dental caries and periodontal pathogens

Imaging projects in the lab include:

  • Bacterial adhesion on collagen membranes: visualization by fluorescent dyes using confocal microscopy.
  • Effecct of the periopathogen Aggregatibacter actinomycetemcomitans toxic factor/s on Streptococcus mutans.   

SCMIC equipment used:

Confocal microscopy: Zeiss 510 Meta – NLO (includes Two-photon LASER); Zeiss 410; Leica SP5

Figure: Salivary bacteria on a collagen membrane. Live bacteria are stained green, dead bacteria are stained red (LIVE/DEAD Backlight Bacterial viability kit: Syto 9 green fluorescent nucleic acid stain, Propidium iodide red fluorescent nucleic acid stain).

Key reference:

Wolff, A., Kozlovsky, A., and Bar-Ness Greensten, R. (2011) Effect of saliva from Aggregatibacter actinomycetemcomitans (Aa) positive, and the effect of Aa spent culture medium, on in vitro growth of Streptococcus mutans. IADR 2011 meeting, Poster.