Prof. Karen B. Avraham

Dept. of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine

email: karena@post.tau.ac.il

 Research topic: Gene discovery, expression and regulation in human hereditary hearing loss.

 Imaging projects in the lab include:

  • Immunofluorescence of mouse inner ears using confocal microscopy.
  • In situ hybridization of embryonic mouse inner ears.   

SCMIC equipment used:

Confocal microscopy: Zeiss 510 Meta Leica SP5

Image analysis services: Deconvolution – Huygens software

Figure: Single mouse inner ear hair cell visualized with phalloidin to label actin (red), myosin VI to label the hair cell cytoplasm (green) and DAPI to label the nucleus (blue). Leica SP5. Shaked Shivatski.

Key references:

Friedman, L.M., Dror, A.A., Mor, E., Tenne, T., Toren, G., Satoh, T., Biesemeier, D.J., Shomron, N., Fekete, D.M., Hornstein, E., and Avraham, K.B. (2009) MicroRNAs are essential for development and function of inner ear hair cells in vertebrates. Proc. Natl. Acad. Sci. USA, 106:7915-7920.

Walsh, T., Pierce, S.B., Lenz, D.R., Brownstein, Z., Dagan-Rosenfeld, O., Shahin, H., Roeb, W., McCarthy, S., Nord, A.S., Gordon, C.R., Ben-Neriah, Z., Sebat, J., Kanaan, M., Lee, M.K., Frydman, M., King, M.-C. and Avraham, K.B. (2010) Genomic duplication and over-expression of TJP2/ZO-2 leads to altered expression of apoptosis genes in progressive non-syndromic hearing loss DFNA51. Am J Hum Genet, 87: 101-109.

Dror, A.A.,Politi, Y., Shahin, H., Lenz, D.R., Dossena, S., Nofziger, C., Fuchs, H., Hrabé de Angelis, M.,Markus, P., Weiner, S. and Avraham, K.B. (2010). Calcium oxalate stone formation in the inner ear as a result of an Slc26a4 mutation. J. Biol. Chem. 285: 21724-21735.

Atar, O. and Avraham, K.B. (2010) Anti-apoptotic factor z-val-ala-asp-fluoromethylketone promotes the survival of cochlear hair cells in a mouse model for human deafness. Neurosci. 168:851–857.