Prof. Ronit Sagi-Eisenberg

Dept. of Cell and Developmental Biology, Sackler Faculty of Medicine


 Research topic: Lysosomal trafficking; lessons from mast cell degranulation and drug-induced autophagy.

 Imaging projects in the lab include:

  • Functional and phenotype driven screen of Rab GTPases in controlling mast cell function using confocal microscopy.
  • Exploring Autophagy as an alternative for cancer treatment.   

SCMIC equipment used: 

Confocal microscopy: Zeiss 510; Zeiss 410; Leica SP5; STAGE incubator

Image analysis services: Deconvolution – Using the Huygens software; 3D and 4D Real-Time Interactive Image Visualization – Using the Imaris software

Figure: Expression of an active mutant of the small GTPase Rab5A in mast cells results in the formation giant secretory granules (SGs). Visualized in this picture are the active GFP-Rab5A mutant (green), which decorates the giant SGs, Neuropeptide Y-mRFP (purple), a fluorescent reporter that is targeted to the SGs, and serotonin, an endogenous resident of the SGs, which is labeled by anti serotonin antibodies (blue). Bar 2mm. By Nurit Pereg.

Key references:

Melnikov S. and Sagi-Eisenberg, R. Down-Regulating Protein Kinase C alpha: Functional Cooperation between the Proteasome and the Endocytic System. Cell Signal. 21, 1607-1619 (2009).

Baram, D., Dekel, O., Mekori, Y. and Sagi-Eisenberg, R. Activation of mast cells by trimeric G protein Gi3; Coupling to A3 adenosine receptor directly and upon T cell contact. J Immunol. 184, 3677-3688 (2010).