Prof. Ronit Satchi-Fainaro

Department of Physiology and Pharmacology, Sackler School of Medicine

Email: ronitsf@post.tau.ac.il

Research topic: Multivalency of polymer therapeutics used for the integration of anti-angiogenic therapy with chemotherapy 

Imaging projects in the lab include:

  • Tumor progression monitoring (fluorescence and bioluminescence).
  • Pharmacokinetics and biodistribution of fluorescently-labeled nanomedicines.
  • Ultrasound imaging of tumor vasculature using contrast media-microbubbles.
  • Characterization of blood vessels using endomicroscopy. 
  • Subcellular trafficking of fluorescently-labeled nanomedicines.

SCMIC equipment used:

Confocal microscopy: Zeiss 510 Meta Zeiss 410 Leica SP5

Whole animal Imaging: Fluorescence Imaging – CRI Maestro; Bioluminescence Imaging – Photon Imager, Biospace; Fiber Confocal Microscopy – CellVizio; Ultrasound: Sequoia 512 (Siemens) and Vevo 2100 (Visual Sonic).

Image analysis services: Deconvolution – Using the Huygens software; 3D and 4D Real-Time Interactive Image Visualization – Using the Imaris software

 

Figure: Tumor-progression monitoring and biodistribution studies using Cri Maestro intravital non-invasive fluorescence imaging system.

Key references:

Segal E, Pan HZ, Udagawa T, Kopečková P, Kopeček J and Satchi-Fainaro R, A novel combined targeted anticancer and anti-angiogenic polymer therapeutic, PLoS One, 4(4):e5233 (2009).

Miller K, Erez R, Segal E, Shabat D and Satchi-Fainaro R, A novel bi-specific agent based on a polymer-alendronate-taxane conjugate to target bone metastases, Angew Chem Int Ed Engl 48, 2949 –2954 (2009).

Ofek P, Fischer W, Calderon M, Haag R and Satchi-Fainaro R, In Vivo delivery of siRNA to tumors and their vasculature by novel dendritic nanocarriers, FASEB Journal24(9), 3122-34 (2010).

Eldar-Boock A, Miller K, Sanchis J, Lupu R, Vicent MJand Satchi-Fainaro R, Integrin-assisted drug delivery of nano-scaled polymer therapeutics bearing paclitaxel, Biomaterials, 32, 3862-3874 (2011).

SegalE, Pan H, Benayoun L, Kopečková P, Shaked Y, Kopeček J and Satchi-Fainaro R,Enhanced safety profile and antitumor activity of targeted HPMA copolymer-alendronate-TNP-470 nanoconjugate in the treatment of bone malignances, Biomaterials, 32, 4450-4463 (2011).

Miller K, Eldar-Boock A, Polyak D,Segal E and Satchi-Fainaro R, Antitumor activity of HPMA copolymer-paclitaxel-alendronate conjugate on breast cancer bone metastases mouse model, Molecular Pharmaceutics, in press (2011).

Karton-Lifshin N, Segal E, Omer L, Portnoy M, Satchi-Fainaro R*, and Shabat D*, A Unique Paradigm for a Turn-ON Near-Infrared Cyanine-Based Probe: Non-Invasive Intravital Optical Imaging of Hydrogen Peroxide, J. Am. Chem. Soc., in press (2011). * Corresponding authors.