Prof. Shimon Efrat

Dept. of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine


Research topic: Beta-cell replacement therapy for diabetes

Imaging projects in the lab:

  • Immunofluorescence of cultured cells and tissue sections using confocal microscopy.

SCMIC equipment used:

Confocal microscopy: Leica SP5

Figure: Cultured cells expanded from human pancreatic islet beta cells labeled green with GFP show nuclear staining with anti-Ki67 antibody (red), demonstrating their active proliferation. By Holger Russ.

Key references:

Russ HA, Bar Y, Ravassard P, Efrat S (2008) In vitro proliferation of cells derived from adult human beta cells revealed by cell-lineage tracing. Diabetes 57:1575 -1583

Bar Y, Russ HA, Knoller S, Ouziel-YahalomL, Efrat S (2008) HES1 is involved in adaptation of adult human beta cells to proliferation in vitro. Diabetes 57:2413-2420.

Russ HA, Ravassard P, Kerr-Conte J, Pattou F, Efrat S (2009) Epithelial-mesenchymal transition in cells expanded in vitro from lineage-traced adult human pancreatic beta cells. PLoS ONE 4: e6417.