Prof. Uri Ashery

Dept. of Neurobiology, Life-Sciences faculty

email: uria@post.tau.ac.il

Research topic: Molecular mechanisms of synaptic function under normal and pathological conditions.

Imaging projects in the lab include:

  • Immunofluorescence of synaptic proteins distribution in the mouse hippocampus.
  • Immunofluorescence expression levels of synaptic proteins in the mouse hippocampus after behavioral manipulations and under pathological conditions.

SCMIC equipment used:

Confocal microscopy: Zeiss 510Meta – includes Two-photon LASER

Tomosyn colocalizes with synaptic and dendritic markers in the mouse hippocampus

Immunofluorescence double-labeling of coronal slices of mouse hippocampus. (A) Tomosyn colocalization with synaptophysin in the stratum lucidum of the mossy fibers. (B) Tomosyn colocalization with VGluT1 in the stratum lucidum of the mossy fibers. (C) Tomosyn colocalization with VGAT in the stratum lucidum of the mossy fibers. (D) Tomosyn colocalization with MAP2 in the stratum radiatum of CA2. (E) Tomosyn colocalization with PSD95 in the stratum radiatum of CA2. PDM, positive product of the differences from the mean; SR, stratum radiatum of CA2; SLu, stratum lucidum of the mossy fibers; IIP, intra- and infrapyramidal of the mossy fibers; Hi, hilus; PY, CA3 pyramidal cells. In positive PDM images, bright spots represent colocalization. Scale bar: A-D = 10 µm, E = 5 m.

Key references:

Barak B, Wang Y, Okun E, Norman E, Sheinin A,Yizhar O, van Praag H, Mattson MP and Ashery U. Hippocampal dentate gyrus neuron-specific tomosyn1 expression enhances learning and memory in mice. Submitted to Nature Communications.

Barak B, Williams A, Bielopolski N, Gottfried I, Okun E, Brown M, Matti U, Rettig J, Stuenkel E and Ashery U. Tomosyn expression and localization at the sub-areas of the mouse hippocampus. Front. Neuroanat. 4:149. doi: 10.3389/fnana.2010.00149