Prof. Yossi Shiloh

Dept. of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine


Research topic: The cellular DNA damage response; the genetic disorder ataxia-telangiectasia

Imaging projects in the lab include:

  • Immunofluorescence analysis of the DNA damage response in a variety of cellular systems. 

SCMIC equipment used:

Confocal microscopy: Zeiss 510 Meta – NLO (includes Two-photon LASER); Zeiss 410; Leica SP5

Key references:

Moyal L, Lerenthal Y, Gana-Weisz M, Mass G, So S, Wang SY, Eppink B, Chung YM, Shalev G, Shema E, Shkedy D, Smorodinsky NI, van Vliet N, Kuster B, Mann M, Ciechanover A, Dahm-Daphi J, Kanaar R, Hu MC, Chen DJ, Oren M, Shiloh Y (2011) Requirement of ATM-Dependent Monoubiquitylation of Histone H2B for Timely Repair of DNA Double-Strand Breaks. Mol Cell 41: 529-542

Segal-Raz H, Mass G, Baranes-Bachar K, Lerenthal Y, Wang S-Y, Chung YM, Ziv-Lehrman S, Strom CE, Helleday T, Hu MCT, Chen DJ, Shiloh Y (2011) ATM-mediated phosphorylation of polynucleotide kinase/phosphatase is required for effective DNA double-strand break repair. EMBO Rep advance online publication

Salton, M., Lerenthal, Y., Wang, S.Y., Chen, D.J., and Shiloh, Y. (2010). Involvement of matrin 3 and SFPQ/NONO in the DNA damage response. Cell Cycle 9, 1568-1576.