Sackler Cellular
and Molecular
Imaging Center

The CRi Maestro imaging system enables multiplexed in vivo fluorescence macro-imaging of small animals with high sensitivity. The system uses an Imaging Module and an Illumination Module for fast and accurate imaging and a liquid crystal element that enables the transmitted light to be electronically tuned. Maestro offers the capability of removing autofluorescence emitted from images of skin and other tissues to reveal otherwise hard-to-detect labeled targets. The dramatic improvement in signal-to-noise can increase sensitivity up to several hundred-fold, enabling much smaller or fainter signals from biological targets to be detected earlier and accurately measured.

The system is equipped with unique multispectral tunable optics and inbuilt multispectral imaging software algorithms which enables users to rapidly image and quantify more than one fluorescent signal within a mouse or rat in real time. The instrument specifications include: View Field of (length x width): 3.9 cm x 2.9 cm to 11.2 cm x 8.4 cm; Heated imaging chamber; Animal isolation containment chambers for up to three mice or one rat; 8 excitation filter sets with wavelength tuning range of 500-950 nm; Maestro software including CPStm (Compute Pure Spectrum) and RCAtm (Real Component Analysis) spectral library generation tools.